商品详情:
Corning 分散酶 是一种细菌来源的中性金属蛋白酶,推荐用于回收培养在Corning基质胶上的细胞。
分子量:36KD
来源:多黏菌衍生金属蛋白酶
检测标准:
-测试在37℃下2小时后溶解1毫米厚的康宁基质胶的能力
-细菌,真菌和支原体检测结果均为阴性
储存:
-20℃可保存两年,避免反复冻融
用法:
推荐使用浓度:10U/cm2 基质胶,(例如:35mm培养皿的基质胶加100U分散酶)
溶剂组分:
HBSS,pH 7.4
Corning Dispase, 100 mL, is a bacillus-derived neutral metalloprotease recommended for recovering cells cultured on Corning Matrigel Basement Membrane Matrix.
Molecular Weight
36kD
Source
Bacillus polymyxa-derived metallo protease
Quality
-Tested for ability to solubilize a 1mm thick layer of gelled Corning Matrigel Matrix after two hours at 37°C
-Tested and found negative for bacteria, fungi, and mycoplasma
Preparation and Storage
Stable for at least three months at -20°C. Avoid multiple freeze-thaws.
Handling
Recommended concentration - 10U/cm² of Corning Matrigel Matrix (e.g., 100U per 35mm dish).
Formulation
Frozen in HBSS, pH 7.4