描述
Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
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Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots
图表
Migration patterns of the PageRuler Prestained Protein Ladder in different electrophoretic conditions
规格
| Detection Method: |
colorimetric, NIR fluorescence (700nm channel), RGB fluorescence (555nm channel) |
| Molecular Weight: |
180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa |
| Number of Markers: |
10 |
| Stain Type: |
3 colors: Blue, Orange, Green |
| Size Range: |
10 to 180 kDa |
| System: |
SDS-PAGE |
| Product Line: |
PageRuler™ |
| Product Size: |
10 x 250 µL |
内容及储存
Contents: 10 vials of 250 µL each
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C